Glycoprotein Staining Assay Kit

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For the staining and detection of glycoproteins on a PVDF or nitrocellulose membrane

MILPITAS, Calif. - eTradeWire -- BioVision's Glycoprotein Staining Kit is designed for the staining and detection of glycoproteins on a PVDF or nitrocellulose membrane using the periodic acid-Schiff (PAS) method. The kit provides all the necessary reagents needed to specifically stain the glycoproteins separated by polyacrylamide gel electrophoresis and transferred to a nitrocellulose or PVDF membrane. The glycoproteins are detected as magenta bands against a colorless background. The kit contains sufficient reagents for staining up to 20 membranes (6 x 9 cm) and can detect as low as 0.5 µg of native glycoproteins.

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Figures A. SDS-PAGE and Coomassie staining of various proteins. B. Selective staining of glycoproteins. Lanes, M: Protein Marker; 1: Deglycosylated recombinant glycoprotein; 2: Recombinant glycoprotein; 3: Positive control (native glycoprotein); 4: Negative control. C. Glycoprotein staining using various amounts of native glycoprotein (10, 5, 2.5, 1 and 0.5 µg respectively). For deglycosylation, 10 µg of glycoprotein (BioVision Cat# P1172) was treated with 0.1 mU of PNGase F (BioVision Cat# P1598) for 2 hr at 37 °C.

For more information on this Assay Kit Cat# K2073, Visit: https://bit.ly/3bz4Jn0

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Source: BioVision, Inc.
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Filed Under: Biotech

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