Popular on eTradeWire


Similar on eTradeWire

EZClick™ Myristoylated Protein Assay Kit, Red Fluorescence

eTradeWire News/10525680
MILPITAS, Calif. - eTradeWire -- Myristoylation is a co- and post-translational modification of proteins. Myristoylated proteins are involved in cellular trafficking, cellular transformation and oncogenesis. In this modification, Myristic acid, a saturated fatty acid is covalently conjugated to a protein through the action of N-myristoyl transferase (NMT). NMT transfers the myristate moiety from Myristoyl-CoA to an N-terminal glycine of a target protein. The NMT enzyme is associated with carcinogenesis. The upregulation of its expression and activity is observed in colorectal cancer, gall-bladder cancer, brain tumor etc.
BioVision offers EZClickTM Myristoylated Protein Assay Kit, a highly specific, simple and robust tool for labeling and detection of myristoylated proteins. In this assay, an Alkynyl Myristic Acid is added directly to the cells that get incorporated into proteins during or post translation followed by the click reaction with the azide-containing dye. This system offers a powerful method for imaging localization, trafficking and dynamics of Myristoylated proteins, or detection by FACS for quantitative studies. Labeled Myristoylated Proteins can be directly detected in 1D or 2D gels using the appropriate excitation sources, or enriched by immunoprecipitation with biotin-azide or antibodies prior to proteomic analysis. We provide sufficient materials for 100 assays in a 96-well plate format.

More on eTradeWire News
Figure:  Jurkat cells: FACS analysis of Negative Control (Black), Background Control (EZClick only, Green), Positive Control (Myristic Acid Label and EZClick, Red). Signal measured in FL-2 channel clearly shows Myristic Acid Labeling of the Protein.
For detailed information on this kit, visit: https://www.biovision.com/ezclicktm-myristoylated-protein-assay-kit-red-fluorescence.html

Media Contact
BioVision Marketing
***@biovision.com
4084931800


Source: BioVision Inc
stats
Filed Under: Biotech

Show All News | Report Violation

0 Comments
1000 characters max.