EZCell™ CFDA SE Cell Tracer Kit

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Flow cytometry based assay to determine cell proliferation using CFDA SE dye

MILPITAS, Calif. - eTradeWire -- Carboxyfluorescein Diacetate Succinimidyl Ester (CFDA SE) is a colorless, non-fluorescent, stable cell-permeable dye. Once it enters into the cells, the intracellular esterases remove the acetate groups to generate Carboxyfluorescein Succinimidyl Ester, which then covalently binds to lysine residues or other amines and produces a bright fluorescent conjugate. The fluorescent conjugate is stable and is retained inside the cells during cell division and hence it is an ideal tool for cell tracing studies, both in vitro and in vivo.

BioVision's EZCell™ CFDA SE Cell Tracer Kit provides an easy, rapid and sensitive way to determine cell proliferation using CFDA SE dye. The dye can be inherited by the daughter cells for up to 8 generations. Following each cell division, the fluorescence intensity is halved, which can be quantified by measuring the fluorescence intensity at Ex/Em = 492/517 nm. The assay is non-radioactive, accurate and can be used for cell tracing studies.

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Figure: Tracking Jurkat Cell Division: Jurkat cells were seeded at 1x106 cells/well into a 6-well plate in complete cell media containing 10% FBS. Cells were either stained with 0.0625 µM CFDA SE or not stained (Negative Control, black Line). Sequential cell divisions were analyzed by flow cytometry using EZCell™ CFDA SE Cell Tracer Kit. Fluorescence intensity was detected and recorded on a BD flow cytometer in FL-1 channel after staining with CFDA SE: 0 hr. (Green line); 24 hr. (Pink line); 48 hr. (Blue line) and 72 hr. (Orange line).

For more information on this product, visit: https://www.biovision.com/ezcelltm-cfda-se-cell-tracer-kit.html

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Source: BioVision Inc
Filed Under: Biotech

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