Beta-Glucan Assay Kit (Fluorometric)

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A simple and sensitive assay for measuring (1, 3)-beta-glucan in various samples.

MILPITAS, Calif. - eTradeWire -- Beta-glucan comprises a group of beta-D-glucose polysaccharides naturally present in the cell walls of grains, bacteria, and fungi. It is a member of the glucan family and a polysaccharide derived from D-glucose, connected by glycosidic bonds. Beta-glucans form a linear backbone with (1-3)-beta-glycosidic bonds, but they vary in molecular mass, solubility, viscosity, branching structure, etc. The presence of (1, 3)-beta-glucan is marketed as a means of identifying invasive or disseminated fungal infections.

BioVision's Beta-Glucan Assay Kit provides a facile, rapid way to monitor the amount of (1, 3)-beta-glucan in various biological samples. In this kit, (1, 3)-beta-glycosidic bonds are cleaved by an enzyme mix to generate glucose. Glucose then reacts with the Beta-Glucan probe and the developer to generate a fluorescent product measured at Ex/Em = 535/587 nm. The assay is simple, sensitive, and can detect as low as 25 ng of (1, 3)-beta-glucan.

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Figures: (a). Beta-Glucan Standard Curve (results from multiple experiments). (b). Percentage of Beta-glucan in mushrooms (50 μg) and yeast (5 μg). All assays were performed following the kit protocol.

For more information on this Assay Kit Cat# K2068, Visit:

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Source: BioVision, Inc.
Filed Under: Biotech

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